genome editing
Checking whether a genome edit worked
How PCR, sequencing and protein checks show whether a genome edit is present, clonal and on target, and which result is still only a hint.
8 October 2026 · 8 min read
Knowledge centre
Methods a laboratory can check.
Guides, method explainers and sourcing notes for research laboratories. Written by the EVRINTH Editorial Team from published methods, with catalogue links for enquiry rather than stock claims.
All topicsBuffers and laboratory water26Cell culture26Chromatography26Cold chain and sample storage26Cytometry and microscopy26DNA sequencing26Gels and blotting26Gene expression26Genome editing26Immunoassays26Laboratory labware26Laboratory safety26Liquid handling26Microbiology26Molecular cloning26Nucleic acid purification26PCR and amplification26Protein biochemistry26Proteomics26Research sourcing and study design26
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520 articles
buffers and water
Chelators EDTA and magnesium-dependent enzymes
Trace a failed magnesium-dependent enzyme to EDTA carry-over, including textbook TE, and decide whether EGTA or less chelator is the check the method actually allows.
8 October 2026 · 8 min read
nucleic acid purification
Choosing a sample input for extraction
Set an extraction input from the assay you will run and the kit capacity class, so too little sample is not missed and too much does not overload.
8 October 2026 · 10 min read
gels and blotting
Choosing agarose percentage for a fragment
Choose an agarose percentage from the expected fragment size and a ladder that brackets it, balancing resolution, gel strength and run time.
8 October 2026 · 9 min read
pcr amplification
Choosing an annealing temperature
Why annealing temperature is not the Tm printed on a tube, and how salt, magnesium, additives, and a short gradient locate the real window.
8 October 2026 · 8 min read
immunoassays
Choosing and checking a primary antibody
How to choose and check a primary antibody: host, clonality, application claims, and controls that show the signal is the intended target.
8 October 2026 · 7 min read
Pillar · labware
Choosing laboratory glassware
How to match borosilicate beakers, flasks and volumetric glass to the measurement you need, and when a printed line is only a guide.
8 October 2026 · 8 min read
dna sequencing
Choosing read length and paired ends
Choose read length and paired or single ends from the sequence you must place. Longer short reads help repeats only a little; mates rescue mapping.
8 October 2026 · 7 min read
gene expression
Choosing reference genes instead of hoping
How to choose reference genes for this experiment by testing stability, instead of trusting a housekeeping name such as GAPDH or ACTB from another study.
8 October 2026 · 9 min read
molecular cloning
Choosing restriction sites that actually cut
Why a restriction site on a plasmid map may not cut the DNA in your tube, and how methylation, star activity and buffer choice decide the digest.
8 October 2026 · 9 min read
proteomics
Chromatography before the mass spectrometer
Reversed-phase C18 is the usual peptide separation before electrospray. Trap-elute or direct injection, and nano versus higher flow, are column specification choices.
8 October 2026 · 8 min read
Pillar · chromatography
Chromatography methods in life-science labs
How life-science chromatography separates molecules on a stationary phase, and what an HPLC or column peak does not prove about purity.
8 October 2026 · 9 min read