proteomics
Reduction alkylation and trypsin
Why proteins are reduced and alkylated before trypsin in bottom-up proteomics, and how incomplete cysteine chemistry changes which peptides a search can name.
8 October 2026 · 11 min read
Knowledge centre
Methods a laboratory can check.
Guides, method explainers and sourcing notes for research laboratories. Written by the EVRINTH Editorial Team from published methods, with catalogue links for enquiry rather than stock claims.
All topicsBuffers and laboratory water26Cell culture26Chromatography26Cold chain and sample storage26Cytometry and microscopy26DNA sequencing26Gels and blotting26Gene expression26Genome editing26Immunoassays26Laboratory labware26Laboratory safety26Liquid handling26Microbiology26Molecular cloning26Nucleic acid purification26PCR and amplification26Protein biochemistry26Proteomics26Research sourcing and study design26
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520 articles
dna sequencing
Reference genomes and why the build matters
Keep the genome build with every coordinate, because GRCh38, hg19, patches, alt contigs and decoys do not share one numbering.
8 October 2026 · 9 min read
nucleic acid purification
Removing PCR inhibitors from difficult samples
Pick dilution, a further cleanup, or an inhibitor-tolerant polymerase when heme, humic acid, heparin or leftover solvent stops a PCR.
8 October 2026 · 9 min read
immunoassays
Reporting an immunoassay so another lab can audit it
An auditable immunoassay file holds lots, the unit, the curve model, raw blanks and excluded wells. A bar on a slide does not replace them.
8 October 2026 · 9 min read
molecular cloning
Restriction ligation and Gibson assembly
How restriction ligation and Gibson assembly join DNA ends, where each method fails, and what colony screening can and cannot confirm.
8 October 2026 · 8 min read
labware
Reusable versus single-use and the waste question
What reusable, single-use, decontamination and recalibration mean when a bench chooses glassware or a disposable plastic format.
8 October 2026 · 7 min read
pcr amplification
Reverse transcription before PCR
Troubleshoot the reverse-transcription step before PCR: two enzymes, primer class, and a no-RT control for genomic DNA. This is not quantification.
8 October 2026 · 8 min read
chromatography
Reversed phase for peptides
Diagnose ruined peptide reversed-phase peaks: hydrophobic retention, acetonitrile gradients, and a sample solvent that is too strong.
8 October 2026 · 7 min read
research sourcing
Rewriting a vague request into a checkable one
Turn a request for some polymerase and tips into fields a quotation can answer: enzyme class, units, storage, tip filter, and water grade.
8 October 2026 · 10 min read
laboratory safety
Risk assessment is a local document
A risk assessment records the agent, the procedure, the people, the place, and the controls. Copying another laboratory's sheet does not create one.
8 October 2026 · 10 min read
nucleic acid purification
RNA extraction on a clean bench
Define RNase, RNase-free plastic, DEPC, ice and dedicated tubes, then see how a shared bench fails an RNA extraction before lysis starts.
8 October 2026 · 9 min read
gene expression
RNA-seq library types polyA and ribodepletion
How poly(A) selection and ribosomal depletion keep different RNA molecules, and when degraded, bacterial or non-polyadenylated samples need the broader library.
8 October 2026 · 8 min read