Skip to content
EVRINTH

Knowledge centre

Methods a laboratory can check.

Guides, method explainers and sourcing notes for research laboratories. Written by the EVRINTH Editorial Team from published methods, with catalogue links for enquiry rather than stock claims.

All topicsBuffers and laboratory water26Cell culture26Chromatography26Cold chain and sample storage26Cytometry and microscopy26DNA sequencing26Gels and blotting26Gene expression26Genome editing26Immunoassays26Laboratory labware26Laboratory safety26Liquid handling26Microbiology26Molecular cloning26Nucleic acid purification26PCR and amplification26Protein biochemistry26Proteomics26Research sourcing and study design26
cold chainbiosafetycrisprspecificationsequencingproteomicsenquiryglossarytroubleshootingcloningmicrobiologybuffersfreezerspipettingcalibrationelisacell cultureglassware

520 articles

Gloved hand pipetting into microtubes kept on ice on a clean stainless RNA workstation

nucleic acid purification

Protecting RNA during extraction

How to keep RNA intact from lysis to elution: RNase control, what A260 ratios do not prove, and the checks that show whether the preparation is still usable.

8 October 2026 · 8 min read

Researcher viewing gene expression heatmaps and genomic tracks on two monitors at night

dna sequencing

Quality scores and a FASTQ file

Read the four lines of a FASTQ record, treat Q20 and Q30 as planning thresholds, and expect quality to fall toward the end of a read.

8 October 2026 · 10 min read

Gloved fingers placing a silica spin column into a collection tube beside a tube rack and pipette tips

nucleic acid purification

Quantifying DNA with a spectrophotometer

A spectrophotometer number is a concentration only after a blank and a pathlength. Compare a pedestal with a cuvette before you trust the reading.

8 October 2026 · 8 min read

Gloved hand reaching into a CO2 incubator stacked with cell culture flasks of red medium

cell culture

Quarantine of a new cell line

A selection guide for quarantining a new cell line: separate housing, mycoplasma and identity checks, a visual inspection, a token freeze, and what risk remains.

8 October 2026 · 8 min read

Gloved hand pulling a rack of cryoboxes from a frosted ultra-low temperature freezer

cold chain

Questions before a cold-storage enquiry

Write volume, temperature class, alarm, backup power, footprint, inventory format, and documents before you enquire. Leave price out.

8 October 2026 · 10 min read

Gloved hand placing a vial into the autosampler of a modular HPLC system with solvent bottles on top

chromatography

Questions before a column enquiry

Write a column enquiry that names chemistry, particle size, dimensions, pressure rating, pH window, matrix, and analytical or prep goal.

8 October 2026 · 7 min read

Glass beakers and graduated cylinders holding blue and clear liquids on a dark lab bench

labware

Questions before a glassware enquiry

Which glass type, class, volume, joint size, cap thread and autoclave requirement to write down before a laboratory glassware enquiry.

8 October 2026 · 7 min read

Close-up of a micropipette tip releasing a droplet into a microcentrifuge tube held in a gloved hand

liquid handling

Questions before a pipette enquiry

List volume range, displacement type, channels, tip family, calibration documents, and chemical exposures before you send a pipette enquiry.

8 October 2026 · 7 min read

Gloved hand reaching into a CO2 incubator stacked with cell culture flasks of red medium

cell culture

Questions before a plasticware enquiry for culture

What to specify before a culture-plastic enquiry: printed growth area, treated or untreated surface, cap type, material, and the cell behaviour the vessel assumes.

8 October 2026 · 9 min read

Mass spectrometer coupled to a liquid chromatography system with sample vials in the foreground

proteomics

Questions before a proteomics enquiry

Questions to write down before a proteomics enquiry: contrast, matrix, replicates, detergents, modifications, database, shipping temperature and the report fields you require.

8 October 2026 · 8 min read

Safety goggles, lab coat, nitrile gloves and a face shield on a bench with an eyewash station behind

laboratory safety

Questions before a safety-equipment enquiry

Before you enquire about a cabinet or a hood, name who it protects, which test documents you will read, and who will maintain it.

8 October 2026 · 9 min read