Knowledge centre

Methods a laboratory can check.

Guides, method explainers and sourcing notes for research laboratories. Written by the EVRINTH Editorial Team from published methods, with catalogue links for enquiry rather than stock claims.

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520 articles

Gloved hands pouring acrylamide solution between glass plates in a gel casting stand

protein biochemistry

Ice buckets and how long a prep really sat out

What to record when a prep is called on ice: clock time, proteases that still work at 4 C, meltwater, and a column stopped by a power cut.

8 October 2026 · 8 min read

chromatography

Imidazole concentration and nickel resin

Set imidazole for a nickel resin from the manufacturer range: a low-millimolar wash and a higher-millimolar elution, not a guess.

8 October 2026 · 7 min read

Gloved hand holding a stained tissue slide beside a rack of stained microscope slides

immunoassays

Immunohistochemistry is a different matrix

Why an antibody that works in an ELISA can fail on fixed tissue, and which controls make an immunohistochemistry result a research observation.

8 October 2026 · 8 min read

Modern research laboratory at dusk with researchers at benches and a city skyline through large windows

research sourcing

Import documents are a compliance question

Define the import papers a laboratory should ask to see, and leave the compliance decision with the institution, the carrier, and the authority.

8 October 2026 · 8 min read

Safety goggles, lab coat, nitrile gloves and a face shield on a bench with an eyewash station behind

laboratory safety

Incident reporting without blame theatre

Incident, exposure, route, and corrective action are different words. This glossary shows what a useful report contains and what blame leaves out.

8 October 2026 · 8 min read

protein biochemistry

Inclusion bodies and refolding as a concept

An inclusion body is insoluble expression, not proof a protein can never fold. Refolding is empirical, and activity rather than a band shows success.

8 October 2026 · 7 min read

Gloved hand reaching into a CO2 incubator stacked with cell culture flasks of red medium

microbiology

Incubator temperature and shaking speed

Routine E. coli cloning cultures are shaken near 37 Celsius so the broth stays aerated. This comparison separates that shaker from a plate incubator and from a CO2 box.

8 October 2026 · 9 min read

genome editing

Indels frameshifts and protein loss

An indel, a frameshift and a missing protein are three claims. Nonsense-mediated decay, exon skipping and extra gene copies keep them apart.

8 October 2026 · 9 min read

Benchtop sequencing instrument with a teal status light and a flow cell cartridge in a genomics lab

dna sequencing

Index collisions in multiplexed pools

Same index twice, indexes one edit apart, and sheets written as the reverse complement. How those collisions misassign reads in a multiplexed pool.

8 October 2026 · 8 min read

Erlenmeyer flasks of amber bacterial culture clamped on an orbital shaker platform

microbiology

Induction temperature and inclusion bodies

Inclusion bodies are insoluble aggregates from overexpression. A cooler induction can leave more protein soluble, and a gel of soluble and pellet fractions shows which.

8 October 2026 · 10 min read

gene expression

Inhibition and weird Cq values

How late, early or impossible Cq values point to inhibition, primer-dimer or a failed control, and which check separates those causes.

8 October 2026 · 9 min read

Gloved hand closing the lid of a benchtop PCR thermal cycler holding a strip of PCR tubes, city lights at dusk behind

pcr amplification

Inhibition of PCR by sample contaminants

Separate a true PCR negative from a dead tube when heme, humic acids, ethanol, guanidine, heparin, bile salts or excess DNA may be present.

8 October 2026 · 8 min read