nucleic acid purification
RNase contamination on shared benches
Skin, dust, old racks and recycled tubes seed RNase on a shared bench. Separate contamination from inhibition, and know when a wipe is not enough.
8 October 2026 · 7 min read
Knowledge centre
Methods a laboratory can check.
Guides, method explainers and sourcing notes for research laboratories. Written by the EVRINTH Editorial Team from published methods, with catalogue links for enquiry rather than stock claims.
All topicsBuffers and laboratory water26Cell culture26Chromatography26Cold chain and sample storage26Cytometry and microscopy26DNA sequencing26Gels and blotting26Gene expression26Genome editing26Immunoassays26Laboratory labware26Laboratory safety26Liquid handling26Microbiology26Molecular cloning26Nucleic acid purification26PCR and amplification26Protein biochemistry26Proteomics26Research sourcing and study design26
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520 articles
gene expression
RT-qPCR for relative expression
How RT-qPCR for relative expression compares transcripts, using reference genes, efficiency checks and controls that keep a fold change honest.
8 October 2026 · 8 min read
gels and blotting
Safety around UV transilluminators
Use the orange shield on a UV transilluminator, and treat eyes, skin, time, and wavelength class under your institution rule.
8 October 2026 · 7 min read
nucleic acid purification
Salting-out and organic extraction concepts
Reason from a dirty phenol interphase or a low 260/230 back to salting-out or organic phase chemistry, without treating solvents as a casual recipe.
8 October 2026 · 10 min read
cytometry and microscopy
Sample filters and clogged nozzles
How to match a sample filter to the nozzle or flow-cell class, and what to do when the event rate collapses from a clog.
8 October 2026 · 8 min read
dna sequencing
Sample identity mix-ups and plate maps
A swapped column or a mismatched index ruins identity before chemistry starts. How plate maps, barcodes and fingerprints catch the swap.
8 October 2026 · 8 min read
gene expression
Sample naming so a bioinformatics file matches the freezer
A naming scheme that keeps freezer tubes, metadata sheets and bioinformatics columns on the same sample, including labels that survive humidity.
8 October 2026 · 10 min read
proteomics
Sample randomisation and batch design
How sample randomisation and blocking stop a processing day from masquerading as biology, and why a confounded proteomics batch often cannot be rescued later.
8 October 2026 · 7 min read
chromatography
Sample solvents that wreck a peak shape
Name the sample-solvent failures that split, front, or break through a peak, and decide whether to dilute before the next injection.
8 October 2026 · 7 min read
immunoassays
Sandwich direct and competitive ELISA
How to choose a direct, indirect, sandwich or competitive ELISA from analyte size, available epitopes and whether signal should rise or fall.
8 October 2026 · 10 min read
molecular cloning
Sanger confirmation of a clone
How to confirm a clone by Sanger sequencing: place the primer upstream, send clean DNA, plan a few hundred reliable bases, and read mixed peaks as mixed template.
8 October 2026 · 8 min read
dna sequencing
Sanger sequencing for a single amplicon
Decide when one cleaned amplicon belongs on a Sanger trace, and how mixed peaks, primer sit-down and dye blobs limit the claim.
8 October 2026 · 10 min read