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EVRINTH

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Methods a laboratory can check.

Guides, method explainers and sourcing notes for research laboratories. Written by the EVRINTH Editorial Team from published methods, with catalogue links for enquiry rather than stock claims.

All topicsBuffers and laboratory water26Cell culture26Chromatography26Cold chain and sample storage26Cytometry and microscopy26DNA sequencing26Gels and blotting26Gene expression26Genome editing26Immunoassays26Laboratory labware26Laboratory safety26Liquid handling26Microbiology26Molecular cloning26Nucleic acid purification26PCR and amplification26Protein biochemistry26Proteomics26Research sourcing and study design26
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520 articles

Close-up of a micropipette tip releasing a droplet into a microcentrifuge tube held in a gloved hand

Pillar · liquid handling

Accurate micropipetting technique

How an air-displacement micropipette delivers a volume, how forward pipetting uses the two stops, and why temperature belongs in the result.

8 October 2026 · 9 min read

Gloved hands aspirating pink culture medium from a cell culture flask inside a laminar flow hood

buffers and water

Acids bases and the fume hood

Dilute a concentrated acid or base for buffer work inside a fume hood, and keep that chemistry out of the laminar-flow hood shown with culture medium.

8 October 2026 · 9 min read

Gloved hands pouring acrylamide solution between glass plates in a gel casting stand

protein biochemistry

Activity assays versus abundance

Milligrams per millilitre are not enzyme units. Specific activity joins rate and mass, and a pure inactive protein differs from a crude active one.

8 October 2026 · 6 min read

Benchtop sequencing instrument with a teal status light and a flow cell cartridge in a genomics lab

dna sequencing

Adapters indexes and barcode hopping

Separate adapter sequence from i5 and i7 indexes, and see why unique dual indexes resist barcode hopping better than one shared index.

8 October 2026 · 8 min read

Gloved hand adjusting the stopcock of a resin-packed chromatography column dripping into collection tubes

chromatography

Affinity tags and competitive elution

Choose an affinity tag class and a competitive elution that the resin protocol supports, then decide which fractions to keep.

8 October 2026 · 7 min read

Agarose gel with glowing DNA bands on a UV transilluminator under an orange safety shield

Pillar · gels and blotting

Agarose gel electrophoresis for DNA

How agarose percentage, buffer and a size ladder turn a DNA sample into a band, and what that band does and does not prove about identity.

8 October 2026 · 8 min read

Gloved hands pouring acrylamide solution between glass plates in a gel casting stand

protein biochemistry

Aggregation and cloudy protein samples

Cloudiness, a spin pellet or a void-volume peak means aggregated protein. Do not treat a turbid sample as a concentration or load it onto a column.

8 October 2026 · 7 min read

Benchtop sequencing instrument with a teal status light and a flow cell cartridge in a genomics lab

dna sequencing

Amplicon sequencing for a defined region

Use amplicon sequencing only for the region the primers define, and see why depth, chimeras and primer dropout are not a genome.

8 October 2026 · 10 min read