liquid handling
Comparing manual and automated handling
See which variances a liquid handler removes and which tip, clot, dead-volume, and deck errors it adds, then plan the check.
8 October 2026 · 7 min read
Knowledge centre
Methods a laboratory can check.
Guides, method explainers and sourcing notes for research laboratories. Written by the EVRINTH Editorial Team from published methods, with catalogue links for enquiry rather than stock claims.
All topicsBuffers and laboratory water26Cell culture26Chromatography26Cold chain and sample storage26Cytometry and microscopy26DNA sequencing26Gels and blotting26Gene expression26Genome editing26Immunoassays26Laboratory labware26Laboratory safety26Liquid handling26Microbiology26Molecular cloning26Nucleic acid purification26PCR and amplification26Protein biochemistry26Proteomics26Research sourcing and study design26
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520 articles
protein biochemistry
Comparing quantification assays for a lysate
Bradford, BCA and absorbance at 280 nm on a dirty lysate: which interference wins, why two kits disagree, and which standard to name.
8 October 2026 · 8 min read
gene expression
Comparing RT-qPCR and RNA-seq on the same question
Why RT-qPCR and RNA-seq can disagree on one gene, and when that disagreement means they measured different molecules rather than a failed assay.
8 October 2026 · 8 min read
cold chain
Comparing storage temperatures for nucleic acids
DNA often tolerates minus 20 better than RNA. Compare fridge, minus 20, and minus 80 against the kit and the sample SOP.
8 October 2026 · 8 min read
research sourcing
Comparing two datasheets without copying them
When two datasheets will not line up, extract the same fields into the laboratory table: analyte, matrix, range, control, storage, and documents.
8 October 2026 · 8 min read
cytometry and microscopy
Compensation is about overlap not brightness
Why compensation subtracts spectral overlap measured from single stains, and why sliding brightness cannot repair a spillover matrix.
8 October 2026 · 7 min read
microbiology
Competent cell handling on ice
How to thaw, hold and plate chemically competent E. coli so the cells stay cold and the colony still reports the DNA you added.
8 October 2026 · 11 min read
molecular cloning
Competent cells and transformation controls
How to choose chemical competence or electroporation, and which plates report efficiency, no-DNA background and a ligation that had no insert.
8 October 2026 · 8 min read
protein biochemistry
Concentrating a dilute protein
How to choose ultrafiltration, precipitation or polymer dialysis when a protein is dilute, and why the smaller volume is not yet a concentration.
8 October 2026 · 8 min read
buffers and water
Conductivity TOC and what each detects
Conductivity and resistivity report ions. TOC reports oxidisable organic carbon. Choose the test that matches the blank your assay can actually fail.
8 October 2026 · 8 min read
cytometry and microscopy
Confocal versus widefield in outline
What a confocal pinhole buys in optical sectioning, and what widefield keeps in speed, photons and gentler light on a thin sample.
8 October 2026 · 7 min read
cell culture
Contamination by bacteria yeast and mycoplasma
How bacteria, yeast and mycoplasma look different in a research culture, which signs you must not trust, and what a testing specification should name.
8 October 2026 · 8 min read