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EVRINTH

Knowledge centre

Methods a laboratory can check.

Guides, method explainers and sourcing notes for research laboratories. Written by the EVRINTH Editorial Team from published methods, with catalogue links for enquiry rather than stock claims.

520 articles

Gloved hand streaking an agar plate with an inoculating loop beside a stack of cultured plates

microbiology

Satellite colonies around a large colony

Why tiny colonies ring a large one on ampicillin, and how that halo differs from kanamycin plates poured for cloning strains.

8 October 2026 · 9 min read

Rack of flow cytometry tubes in front of a researcher viewing coloured scatter plots beside a cytometer

cytometry and microscopy

Scale bars and what an image can claim

How a calibrated scale bar, a linear lookup table and an unsaturated image decide what a research fluorescence figure may claim.

8 October 2026 · 7 min read

Gloved hand placing a vial into the autosampler of a modular HPLC system with solvent bottles on top

chromatography

Scaling a prep from a scout column

Scale a prep from a scout column by holding bed height and linear velocity, scaling load with volume, and not copying pressure.

8 October 2026 · 7 min read

Gloved hands pouring acrylamide solution between glass plates in a gel casting stand

gels and blotting

SDS-PAGE for protein size estimates

Plan an SDS-PAGE size estimate: stacking and resolving gels, SDS and reduction, apparent mass, and a ladder you do not read past.

8 October 2026 · 9 min read

Close-up of a micropipette tip releasing a droplet into a microcentrifuge tube held in a gloved hand

liquid handling

Serological pipettes are a different tool

Tell a blow-out serological pipette from a micropipette, use a controller, and retire a cracked pipette before it delivers a wrong millilitre.

8 October 2026 · 7 min read

Mass spectrometer coupled to a liquid chromatography system with sample vials in the foreground

cell culture

Serum-free media as a concept

What serum-free and chemically defined media claim, why a sudden swap selects or kills cells, and how to adapt a line with a serum-grown backup.

8 October 2026 · 9 min read

Close-up of a micropipette tip releasing a droplet into a microcentrifuge tube held in a gloved hand

laboratory safety

Sharps and glass pasteur pipettes

A glass Pasteur pipette is a sharp in the middle of an ordinary transfer. Substitution and the waste stream are institutional decisions.

8 October 2026 · 9 min read

Gloved fingers placing a silica spin column into a collection tube beside a tube rack and pipette tips

nucleic acid purification

Silica columns versus magnetic beads

Choose a silica column or magnetic beads from debris load, plate throughput and fragment length, since both often share the same bind-wash-elute chemistry.

8 October 2026 · 9 min read