Skip to content
EVRINTH

Knowledge centre

Methods a laboratory can check.

Guides, method explainers and sourcing notes for research laboratories. Written by the EVRINTH Editorial Team from published methods, with catalogue links for enquiry rather than stock claims.

520 articles

Gloved hand holding a tablet in front of shelves of laboratory consumables and reagents

research sourcing

What a quotation can and cannot promise

Check a quotation for the scope, documents, and acceptance test it writes down, and treat an unstated date, performance, or result as not yet agreed.

8 October 2026 · 9 min read

Open stainless steel autoclave with a wire basket of glass media bottles inside

laboratory safety

What an autoclave indicator does not prove

Tape, a chemical integrator, and a biological indicator answer different questions. Choose the class that matches the claim you need about a cycle.

8 October 2026 · 10 min read

Gloved hands pouring acrylamide solution between glass plates in a gel casting stand

protein biochemistry

What an extinction coefficient assumes

A composition-based extinction coefficient is a conversion with conditions attached. Tags, cofactors and nucleic acid change the number you may report.

8 October 2026 · 6 min read

Erlenmeyer flasks of amber bacterial culture clamped on an orbital shaker platform

microbiology

What blue and white colonies can and cannot mean

Blue and white colonies report alpha-complementation, which is a screen. A white colony still needs a digest or a sequence before anyone calls the insert correct.

8 October 2026 · 9 min read

Gloved hand closing the lid of a benchtop PCR thermal cycler holding a strip of PCR tubes, city lights at dusk behind

pcr amplification

What dNTPs magnesium and polymerase each do

Change dNTPs, magnesium, or polymerase one variable at a time, and treat proofreading fidelity as a cloning choice rather than a brighter band.

8 October 2026 · 8 min read

Benchtop sequencing instrument with a teal status light and a flow cell cartridge in a genomics lab

genome editing

What sequence data to request after an edit

After an edit, request coordinates, the genome build, FASTQ files, an allele table, coverage and controls. A PDF of the on-target site is not the dataset.

8 October 2026 · 8 min read

Gloved hand inserting a cuvette of blue solution into a UV-Vis spectrophotometer

dna sequencing

What to check before a sequencing run

What to confirm before a sequencing run: sample identity, amount, fragment size, indexes, and which failed check should stop the instrument.

8 October 2026 · 8 min read

Benchtop sequencing instrument with a teal status light and a flow cell cartridge in a genomics lab

dna sequencing

What to put in a sequencing statement of work

The lines a sequencing statement of work needs: organism, geometry, depth, reference build, files, retention and the rule for a failed sample.

8 October 2026 · 7 min read

Gloved hand pipetting into a tube near a cold block and agar plates with colonies, gel image on a monitor

molecular cloning

What to send for a gene synthesis enquiry

See which missing field blocks a gene-synthesis quotation: sequence, vector, host, sites, expression limits, and the biosafety of the DNA.

8 October 2026 · 7 min read

Gloved fingers placing a silica spin column into a collection tube beside a tube rack and pipette tips

nucleic acid purification

When to repeat an extraction

Compare repeating the extraction, diluting and retrying the assay, and cleaning the same eluate. Use blanks, labels, yield and debris to choose.

8 October 2026 · 7 min read

Stacked boxes of pipette tips in blue racks

labware

When to use plastic tubes tips and plates

When polypropylene tubes, pipette tips and polystyrene plates are the right vessel, and when solvent or volume still belongs in glass.

8 October 2026 · 8 min read