Skip to content
EVRINTH

Knowledge centre

Methods a laboratory can check.

Guides, method explainers and sourcing notes for research laboratories. Written by the EVRINTH Editorial Team from published methods, with catalogue links for enquiry rather than stock claims.

All topicsBuffers and laboratory water26Cell culture26Chromatography26Cold chain and sample storage26Cytometry and microscopy26DNA sequencing26Gels and blotting26Gene expression26Genome editing26Immunoassays26Laboratory labware26Laboratory safety26Liquid handling26Microbiology26Molecular cloning26Nucleic acid purification26PCR and amplification26Protein biochemistry26Proteomics26Research sourcing and study design26
cold chainbiosafetycrisprspecificationsequencingproteomicsenquiryglossarytroubleshootingcloningmicrobiologybuffersfreezerspipettingcalibrationelisacell cultureglassware

520 articles

Wall-mounted water purification unit dispensing ultrapure water into a glass flask

buffers and water

Questions before a water-system enquiry

Write a water-system enquiry that names the feed, the daily volume, the contaminant that matters, pretreatment, sanitisation, and the documents the laboratory will check.

8 October 2026 · 9 min read

Gloved hand sliding a yellow-developed ELISA plate into a microplate reader drawer

immunoassays

Questions before an antibody enquiry

Target, species, application, immunogen, host, conjugate and storage on arrival. Each question stops a different wrong reagent from being quoted.

8 October 2026 · 10 min read

Visualisation of a protein complex binding a DNA double helix, representing genome editing

genome editing

Questions before an editing-validation enquiry

An editing-validation enquiry needs cell type, delivery class, guide sequences, genome build, locus, sample count and the readout you want. A gene name alone is not enough.

8 October 2026 · 7 min read

Mass spectrometer coupled to a liquid chromatography system with sample vials in the foreground

microbiology

Questions before an enzyme or media enquiry

A useful enzyme or media enquiry names the strain, the recognition or recipe class, the antibiotic, and the documents you need. This page is that checklist.

8 October 2026 · 8 min read

Rack of flow cytometry tubes in front of a researcher viewing coloured scatter plots beside a cytometer

cytometry and microscopy

Questions before an instrument enquiry

Which lasers, detectors, filters, formats and sample constraints to answer before you ask for a cytometer or microscope quotation.

8 October 2026 · 7 min read

Researcher viewing gene expression heatmaps and genomic tracks on two monitors at night

gene expression

Questions before an RNA-seq enquiry

The scientific questions to settle before an RNA-seq enquiry: organism, library chemistry, depth, replicates, integrity and the analysis you want.

8 October 2026 · 9 min read

Agarose gel with glowing DNA bands on a UV transilluminator under an orange safety shield

gels and blotting

Questions before ordering gel reagents

Questions on agarose grade, acrylamide hazard, membrane pore, ladder range, and buffer class before you send a gel-reagent enquiry.

8 October 2026 · 8 min read

Gloved fingers placing a silica spin column into a collection tube beside a tube rack and pipette tips

nucleic acid purification

Questions for a nucleic acid prep enquiry

Ask sample type, DNA or RNA, the downstream method, inhibitor risk, throughput and documentation before you send a nucleic acid prep enquiry.

8 October 2026 · 7 min read

Gloved hands pouring acrylamide solution between glass plates in a gel casting stand

protein biochemistry

Questions for a protein expression enquiry

The sequence, tag, host, modification, purity evidence, endotoxin need and assay buffer a protein expression enquiry has to carry.

8 October 2026 · 9 min read

Benchtop sequencing instrument with a teal status light and a flow cell cartridge in a genomics lab

dna sequencing

Questions for a Sanger versus NGS decision

Questions that separate a Sanger trace from a short-read pool: one amplicon, a mixture, a haplotype, a plasmid junction. Turnaround is asked, not promised.

8 October 2026 · 8 min read