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EVRINTH

Knowledge centre

Methods a laboratory can check.

Guides, method explainers and sourcing notes for research laboratories. Written by the EVRINTH Editorial Team from published methods, with catalogue links for enquiry rather than stock claims.

All topicsBuffers and laboratory water26Cell culture26Chromatography26Cold chain and sample storage26Cytometry and microscopy26DNA sequencing26Gels and blotting26Gene expression26Genome editing26Immunoassays26Laboratory labware26Laboratory safety26Liquid handling26Microbiology26Molecular cloning26Nucleic acid purification26PCR and amplification26Protein biochemistry26Proteomics26Research sourcing and study design26
cold chainbiosafetycrisprspecificationsequencingproteomicsenquiryglossarytroubleshootingcloningmicrobiologybuffersfreezerspipettingcalibrationelisacell cultureglassware

520 articles

Scientist in a lab coat writing in a lab notebook beside printed reports and a laptop

research sourcing

Commissioning a sequencing or proteomics study

Commission sequencing or proteomics by locking the question, the reference database, sample QC and the deliverable before a library or a digest is made.

8 October 2026 · 10 min read

Gloved hands pouring acrylamide solution between glass plates in a gel casting stand

gels and blotting

Comparing agarose and polyacrylamide

Choose agarose or polyacrylamide from the molecule and the size, including tank geometry, resolution, and the monomer hazard.

8 October 2026 · 7 min read

Wall-mounted water purification unit dispensing ultrapure water into a glass flask

buffers and water

Comparing bottled water and a point-of-use system

Choose bottled molecular-biology water or a point-of-use polisher for PCR, chromatography, or cell-culture reagents, and see how each one fails after it leaves the maker.

8 October 2026 · 9 min read

Petri dish with blue and white bacterial colonies for blue-white screening

microbiology

Comparing chemical and electrocompetent cells

Chemical competence uses salt and a heat shock. Electrocompetence uses a low-salt cell and a pulse. The DNA cleanup has to match the method you chose.

8 October 2026 · 9 min read

Gloved hand pipetting into a tube near a cold block and agar plates with colonies, gel image on a monitor

molecular cloning

Comparing cloning methods for a short insert

Pick restriction ligation, Gibson assembly, Golden Gate, or annealed oligos for a short insert using length, scars, and an orientation check.

8 October 2026 · 8 min read

Mass spectrometer coupled to a liquid chromatography system with sample vials in the foreground

proteomics

Comparing DIA and DDA for a first study

Choosing DIA or DDA for a first study of twelve samples and two conditions, including missing values, a predicted library and what a power cut does to the queue.

8 October 2026 · 7 min read

Gloved hands lifting a western blot membrane with forceps over a buffer tray, rocking shaker behind

immunoassays

Comparing ELISA and western blot evidence

ELISA returns a number when the curve holds. A blot returns a band at a mass. Their disagreements point at epitope, matrix, or the hook effect.

8 October 2026 · 10 min read

Gloved hand reaching into a CO2 incubator stacked with cell culture flasks of red medium

cell culture

Comparing flasks dishes and plates

How to choose flasks, dishes or multiwell plates for a culture week: expansion, treatment and imaging, with the failure each format is prone to.

8 October 2026 · 9 min read

Gloved hand placing a vial into the autosampler of a modular HPLC system with solvent bottles on top

chromatography

Comparing HPLC and open-column work

Decide when HPLC pressure and resolution are worth it, and when a gravity column and larger fractions are the honest tool.

8 October 2026 · 7 min read

Visualisation of a protein complex binding a DNA double helix, representing genome editing

genome editing

Comparing knockout and knockdown evidence

A knockout is a permanent DNA allele backed by orthogonal evidence. A knockdown only lowers the transcript. A shift in Cq does not establish a knockout.

8 October 2026 · 7 min read