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EVRINTH

Knowledge centre

Methods a laboratory can check.

Guides, method explainers and sourcing notes for research laboratories. Written by the EVRINTH Editorial Team from published methods, with catalogue links for enquiry rather than stock claims.

All topicsBuffers and laboratory water26Cell culture26Chromatography26Cold chain and sample storage26Cytometry and microscopy26DNA sequencing26Gels and blotting26Gene expression26Genome editing26Immunoassays26Laboratory labware26Laboratory safety26Liquid handling26Microbiology26Molecular cloning26Nucleic acid purification26PCR and amplification26Protein biochemistry26Proteomics26Research sourcing and study design26
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520 articles

Open stainless steel autoclave with a wire basket of glass media bottles inside

labware

Racks that survive an autoclave

Why an autoclave rack warps or melts, and how the marking separates polypropylene that can take steam from polystyrene that cannot.

8 October 2026 · 7 min read

Gloved hands pouring acrylamide solution between glass plates in a gel casting stand

Pillar · protein biochemistry

Reading a protein gel

How to read a protein gel: what SDS-PAGE bands, smears, ladders and loading differences can support, and what they cannot identify.

8 October 2026 · 8 min read

Agarose gel with glowing DNA bands on a UV transilluminator under an orange safety shield

pcr amplification

Reading an agarose gel after PCR

Use lane order, marker range, expected size, and the no-template control to decide whether a post-PCR agarose band is usable.

8 October 2026 · 8 min read

Agarose gel with glowing DNA bands on a UV transilluminator under an orange safety shield

gels and blotting

Reading ladders bands and smears

Read a DNA gel by the ladder on that photograph: log spacing, thick bands, doublets, RNA smears and primer-dimer at the dye front.

8 October 2026 · 9 min read

Gloved hand pulling a rack of cryoboxes from a frosted ultra-low temperature freezer

cold chain

Receiving a warm parcel

A warm parcel stays out of inventory until you have named the shipper, the coolant, any excursion, and the chain of custody.

8 October 2026 · 10 min read

Gloved hand sliding a yellow-developed ELISA plate into a microplate reader drawer

immunoassays

Recombinant versus polyclonal reagents

Polyclonal, hybridoma monoclonal and recombinant antibodies differ in epitope count and lot definition. Choose the class in the specification, not by slogan.

8 October 2026 · 9 min read

Visualisation of a protein complex binding a DNA double helix, representing genome editing

genome editing

Recording guides so an experiment can be repeated

Record the spacer 5-prime to 3-prime, the enzyme, the PAM, the assembly, coordinates, strand, modifications, plasmid map and lot so someone else can reorder the guide.

8 October 2026 · 7 min read

Scientist in a lab coat writing in a lab notebook beside printed reports and a laptop

cell culture

Records a culture notebook should hold

The culture notebook fields that let someone else continue a line: identity, passage, medium lot, observations, contamination checks and the freezer location.

8 October 2026 · 7 min read

Modern research laboratory at dusk with researchers at benches and a city skyline through large windows

research sourcing

Records to keep after a delivery

Record what arrived, the temperature evidence, the documents, the printed lot, the storage place, and the acceptance check after a delivery.

8 October 2026 · 10 min read

Gloved hands pouring acrylamide solution between glass plates in a gel casting stand

protein biochemistry

Reducing agents and disulfide bonds

DTT, beta-mercaptoethanol and TCEP break disulfides in different ways. Reducing and non-reducing gels ask whether oligomers are covalently linked.

8 October 2026 · 8 min read