microbiology
Streaking for single colonies
A streak dilutes cells across agar until a sector holds colonies that each grew from one cell. This glossary names the pattern, the loop, and a smear.
8 October 2026 · 9 min read
Knowledge centre
Methods a laboratory can check.
Guides, method explainers and sourcing notes for research laboratories. Written by the EVRINTH Editorial Team from published methods, with catalogue links for enquiry rather than stock claims.
All topicsBuffers and laboratory water26Cell culture26Chromatography26Cold chain and sample storage26Cytometry and microscopy26DNA sequencing26Gels and blotting26Gene expression26Genome editing26Immunoassays26Laboratory labware26Laboratory safety26Liquid handling26Microbiology26Molecular cloning26Nucleic acid purification26PCR and amplification26Protein biochemistry26Proteomics26Research sourcing and study design26
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520 articles
gels and blotting
Stripping a blot and why it can fail
Decide whether to strip a western blot or rerun it. A harsh strip can remove the antigen, and a mild strip can leave the first signal behind.
8 October 2026 · 8 min read
molecular cloning
Synthetic DNA versus PCR-amplified inserts
Choose a synthetic fragment or a PCR copy by polymerase error, codon changes, and the checks both still need before the sequence is trusted.
8 October 2026 · 7 min read
genome editing
T7 endonuclease and amplicon sequencing
T7 endonuclease cuts heteroduplexes from mixed alleles. Amplicon sequencing names bases. A uniform mutant clone can look negative on the gel assay.
8 October 2026 · 9 min read
proteomics
Targeted MRM versus discovery runs
When to screen with a discovery run and when to confirm with targeted MRM: peptide choice, transitions, a matrix blank and a calibration or labelled standard.
8 October 2026 · 8 min read
buffers and water
Temperature compensation on a pH meter
If Tris pH still moves while ATC is on, the meter corrected the electrode slope. The buffer's own chemistry is a separate temperature effect.
8 October 2026 · 8 min read
liquid handling
Temperature of the liquid changes the volume
Equilibrate cold liquids and warm pipettes before you trust an air-displacement volume, and separate that error from a weighing drift.
8 October 2026 · 8 min read
research sourcing
Tender language for a public laboratory
Write tender requirements more than one responsive bidder could meet, and separate mandatory performance from a named example with a stated equivalence test.
8 October 2026 · 8 min read
cell culture
Thawing a vial without cooking it
How to thaw a research cell vial quickly, dilute the cryoprotectant at once, and catch a label, shipment or biosafety problem before the cells are seeded.
8 October 2026 · 7 min read
gene expression
The MIQE idea in everyday language
What a qPCR report should disclose so another scientist can judge the number: sample handling, reverse transcription, primers, efficiency, reference genes and the statistic.
8 October 2026 · 10 min read
pcr amplification
Thermal cycler checks after a power cut
Check whether a thermal cycler finished, restarted at cycle one, or lost its heated-lid set-point before you trust a precious plate.
8 October 2026 · 8 min read
liquid handling
Tip fit is part of the measurement
Write a tip specification that names the cone, the family the pipette was tested with, and what a universal claim does not prove.
8 October 2026 · 7 min read