liquid handling
Troubleshooting scattered replicates
Name the technique, tip, temperature, mixing, and evaporation causes of scattered replicates, and what a method specification should record.
8 October 2026 · 7 min read
Knowledge centre
Methods a laboratory can check.
Guides, method explainers and sourcing notes for research laboratories. Written by the EVRINTH Editorial Team from published methods, with catalogue links for enquiry rather than stock claims.
All topicsBuffers and laboratory water26Cell culture26Chromatography26Cold chain and sample storage26Cytometry and microscopy26DNA sequencing26Gels and blotting26Gene expression26Genome editing26Immunoassays26Laboratory labware26Laboratory safety26Liquid handling26Microbiology26Molecular cloning26Nucleic acid purification26PCR and amplification26Protein biochemistry26Proteomics26Research sourcing and study design26
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520 articles
cell culture
Troubleshooting slow growth
A selection guide for a culture growing more slowly than its own record: which clue to check first, and which reagent class to change only after that check.
8 October 2026 · 8 min read
proteomics
Troubleshooting sparse identifications
Sparse proteomics identifications: check peptide amount, chromatogram shape, detergent humps, the search database, cysteine chemistry and whether only keratin remains.
8 October 2026 · 8 min read
cell culture
Troubleshooting sudden death of a culture
How to name an overnight culture crash: contamination, toxicity, or a temperature excursion, and the first question each appearance of the flask should force.
8 October 2026 · 8 min read
buffers and water
Type 1 type 2 and feed water in plain language
Decide which water name belongs on the method: an ASTM type, an ISO grade, or the feed entering the skid, and which test that name covers.
8 October 2026 · 10 min read
laboratory safety
UV light on a transilluminator
Eye pain after a gel, a shield left up, and DNA that will not clone are different symptoms of the same lamp. The shield and the interlock are the controls.
8 October 2026 · 10 min read
cold chain
Vapour phase versus liquid phase storage
Liquid-phase nitrogen is colder and steadier but submerges tubes. Vapour phase avoids immersion and varies with height and lid openings.
8 October 2026 · 8 min read
dna sequencing
Variant calling is a model not a fact
Treat each VCF row as a model after alignment, and decide when a germline or somatic call still needs an orthogonal Sanger check.
8 October 2026 · 9 min read
molecular cloning
Vector backbone toxicity
Separate leaky expression, a toxic insert, leftover ccdB, and a high-copy burden, then pick a backbone, promoter, or host to test.
8 October 2026 · 8 min read
cold chain
Ventilation when dry ice is in a small room
Dry ice in a small room releases carbon dioxide that pools low. Ventilate. This page is not a clearance to keep dry ice in an office.
8 October 2026 · 9 min read
cytometry and microscopy
Viability dyes and dead-cell artefacts
How dead cells bind antibody nonspecifically, and which amine-reactive or DNA-dye class fits a fixed or unfixed staining workflow.
8 October 2026 · 7 min read
liquid handling
Viscous liquids and slow plunger work
Slow the plunger for viscous liquids, choose reverse or positive displacement, and decide what to do when a film stays in the tip.
8 October 2026 · 8 min read