pcr amplification
Cleaning an amplicon before sequencing
Choose a column, bead or gel cleanup so leftover primers and dNTPs do not wreck the sequencing read you are about to request.
8 October 2026 · 7 min read
Knowledge centre
Methods a laboratory can check.
Guides, method explainers and sourcing notes for research laboratories. Written by the EVRINTH Editorial Team from published methods, with catalogue links for enquiry rather than stock claims.
All topicsBuffers and laboratory water26Cell culture26Chromatography26Cold chain and sample storage26Cytometry and microscopy26DNA sequencing26Gels and blotting26Gene expression26Genome editing26Immunoassays26Laboratory labware26Laboratory safety26Liquid handling26Microbiology26Molecular cloning26Nucleic acid purification26PCR and amplification26Protein biochemistry26Proteomics26Research sourcing and study design26
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520 articles
chromatography
Cleaning and storing a column
Clean and store a column from its insert: salt and organic washes, then the storage solvent that insert names, not a shared cocktail.
8 October 2026 · 7 min read
buffers and water
CO2 and why pH drifts in a carboy
When carboy pH falls overnight, follow dissolved carbon dioxide and buffer capacity first, then decide whether the electrode is the failed control.
8 October 2026 · 8 min read
cell culture
CO2 incubators humidity and pH of medium
Why bicarbonate medium, incubator carbon dioxide and the humidity pan set the pH and the salt concentration your cells actually experience.
8 October 2026 · 9 min read
cell culture
Coating plates for adherent cells
Why some adherent lines need a matrix coating, and how to tell a skipped coat, a dried coat, the wrong plastic or leftover enzyme from a growth problem.
8 October 2026 · 8 min read
protein biochemistry
Codon use and insoluble expression
Why rare codons can slow translation, and why a codon table still does not prove that insoluble expression is a coding problem.
8 October 2026 · 7 min read
research sourcing
Cold-chain instructions belong in the enquiry
Put the cold-chain setpoint, coolant class, logger, excursion owner, and receiving hours into the enquiry before anyone quotes the shipment.
8 October 2026 · 8 min read
pcr amplification
Colony PCR for screening clones
Screen colonies with flanking or insert primers, a lysis step, an empty-vector colony, and a no-template control. A band is not a sequence.
8 October 2026 · 9 min read
molecular cloning
Colony PCR versus diagnostic digest
When to triage colonies by PCR and when a miniprep digest is worth it, including false positives from agar DNA, orientation, and why neither is a sequence.
8 October 2026 · 8 min read
microbiology
Colony picking without mixing clones
How to move one isolated colony of a cloning strain into one labelled tube so the miniprep is not a mixture of neighbours.
8 October 2026 · 9 min read
nucleic acid purification
Column binding capacity and overload
More lysate can lower recovery once a column is past capacity. Read slow flow, dirty ratios and a pellet that will not clear, then split the load.
8 October 2026 · 7 min read
chromatography
Column volume flow rate and pressure
Judge whether a column flow is safe: column volume, linear velocity, residence time, and backpressure as a health signal.
8 October 2026 · 8 min read