gene expression
Single-cell RNA-seq in outline
What single-cell RNA-seq adds beyond a bulk average: droplets or wells, empty droplets, doublets, UMIs, sparse counts, and clustering as a parameter.
8 October 2026 · 7 min read
Knowledge centre
Methods a laboratory can check.
Guides, method explainers and sourcing notes for research laboratories. Written by the EVRINTH Editorial Team from published methods, with catalogue links for enquiry rather than stock claims.
All topicsBuffers and laboratory water26Cell culture26Chromatography26Cold chain and sample storage26Cytometry and microscopy26DNA sequencing26Gels and blotting26Gene expression26Genome editing26Immunoassays26Laboratory labware26Laboratory safety26Liquid handling26Microbiology26Molecular cloning26Nucleic acid purification26PCR and amplification26Protein biochemistry26Proteomics26Research sourcing and study design26
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520 articles
cytometry and microscopy
Single-stain controls
How to build single-stain controls that are bright enough for compensation, and when to branch to cells, beads, or a fresh tube.
8 October 2026 · 8 min read
chromatography
Size-exclusion chromatography concepts
Plan a size-exclusion run that separates aggregates from monomer, and decide what sample volume that bed can honestly accept.
8 October 2026 · 9 min read
gene expression
Small RNA sequencing overview
Why small-RNA libraries fail when adapter dimers dominate, how miRNA arm bias and reference mapping differ from mRNA analysis, and how degradation can imitate a true small RNA.
8 October 2026 · 9 min read
liquid handling
Small volumes near the bottom of the range
Diagnose noisy results at the bottom of a pipette range, move to a lower-range instrument, and watch pre-wetting and the first stop.
8 October 2026 · 7 min read
pcr amplification
Smears and extra bands in PCR
Decide when a smeared or multi-band PCR lane still has a cuttable product, and when cycles, template, temperature, or magnesium call for a redesign.
8 October 2026 · 7 min read
gels and blotting
Smiling bands and uneven gels
Define smile, frown, smear, and diffusion, and trace each band shape to heat, salt, overload, pour, buffer level, or speed.
8 October 2026 · 7 min read
immunoassays
Sodium azide and downstream live-cell work
Sodium azide preserves many antibody stocks, inhibits peroxidase, and poisons cells. Check the vial before an HRP plate or a live culture.
8 October 2026 · 9 min read
chromatography
Solvent quality and ghost peaks
Separate a ghost peak from a sample peak with a blank gradient, and judge water, solvent, and plastic leachables before you integrate.
8 October 2026 · 7 min read
gels and blotting
Southern blot concepts for DNA
Understand a Southern blot as digest, separation, denaturation, transfer and a probe washed at a stringency that matches the question. Not a diagnostic test.
8 October 2026 · 8 min read
gene expression
Spatial transcriptomics as a concept
How spatial transcriptomics attaches expression to a tissue coordinate, why spot and cell resolution differ, and what section quality and permeabilization decide.
8 October 2026 · 9 min read
research sourcing
Specifications that describe performance not a rumour
State purity class, temperature class, the document set, and an acceptance test so a quotation can be checked against performance the laboratory can measure.
8 October 2026 · 10 min read